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First published online June 6, 2008; 10.1104/pp.108.118919 Plant Physiology 147:2041-2053 (2008) © 2008 American Society of Plant Biologists The Transcription Factor VvMYB5b Contributes to the Regulation of Anthocyanin and Proanthocyanidin Biosynthesis in Developing Grape Berries1,2,[W]Institut des Sciences de la Vigne et du Vin, UMR 1287 Ecophysiologie et Génomique Fonctionnelle de la Vigne INRA, Université de Bordeaux 1, Université Victor Ségalen Bordeaux 2, Centre INRA de Bordeaux, 33883 Villenave d'Ornon, France (L.D., T.F., F.B.); CSIRO Plant Industry, Glen Osmond, South Australia 5064, Australia (J.B., A.R.W., S.P.R.); and Groupe d'Etude des Substances Végétales à Activité Biologique, EA 3675, Institut des Sciences de la Vigne et du Vin, Université Victor Segalen Bordeaux 2, UFR Sciences Pharmaceutiques, 33076 Bordeaux cedex, France (A.D., J.-M.M.)
Among the dramatic changes occurring during grape berry (Vitis vinifera) development, those affecting the flavonoid pathway have provoked a number of investigations in the last 10 years. In addition to producing several compounds involved in the protection of the berry and the dissemination of the seeds, final products of this pathway also play a critical role in berry and wine quality. In this article, we describe the cloning and functional characterization of VvMYB5b, a cDNA isolated from a grape berry (V. vinifera ‘Cabernet Sauvignon’) library. VvMYB5b encodes a protein belonging to the R2R3-MYB family of transcription factors and displays significant similarity with VvMYB5a, another MYB factor recently shown to regulate flavonoid synthesis in grapevine. The ability of VvMYB5a and VvMYB5b to activate the grapevine promoters of several structural genes of the flavonoid pathway was confirmed by transient expression of the corresponding cDNAs in grape cells. Overexpression of VvMYB5b in tobacco (Nicotiana tabacum) leads to an up-regulation of genes encoding enzymes of the flavonoid pathway and results in the accumulation of anthocyanin- and proanthocyanidin-derived compounds. The ability of VvMYB5b to regulate particularly the anthocyanin and the proanthocyanidin pathways is discussed in relation to other recently characterized MYB transcription factors in grapevine. Taken together, data presented in this article give insight into the transcriptional mechanisms associated with the regulation of the flavonoid pathway throughout grape berry development.
1 This work was supported by grants from the "Conseil Interprofessionnel du Vin de Bordeaux," The Grape and Wine Research and Development Corporation and the Cooperative Research Centre for Viticulture, and the GABI-Future program of the German Ministry of Education and Research. 2 This article is dedicated to the memory of our friend and colleague, Professor Saïd Hamdi. 3 These authors contributed equally to the article. 4 Present address: Department of Biochemistry, University of Nevada, 1664 North Virginia St., Reno, NV 89557. 5 Present address: Heidelberger Institut für Pflanzenwissenschaften, 69120 Heidelberg, Germany. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: François Barrieu (francois.barrieu{at}bordeaux.inra.fr). [W] The online version of this article contains Web-only data. www.plantphysiol.org/cgi/doi/10.1104/pp.108.118919 * Corresponding author; e-mail francois.barrieu{at}bordeaux.inra.fr. Received March 14, 2008; accepted May 28, 2008; published June 6, 2008. This article has been cited by other articles:
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