This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 148/1/402    most recent pp.108.121400v2 pp.108.121400v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Besserer, A. Articles by Séjalon-Delmas, N. Search for Related Content PubMed PubMed Citation Articles by Besserer, A. Articles by Séjalon-Delmas, N. Agricola Articles by Besserer, A. Articles by Séjalon-Delmas, N.

PLANTS INTERACTING WITH OTHER ORGANISMS

GR24, a Synthetic Analog of Strigolactones, Stimulates the Mitosis and Growth of the Arbuscular Mycorrhizal Fungus Gigaspora rosea by Boosting Its Energy Metabolism^[C],[W]

Plant Cell Surfaces and Signaling Laboratory, UMR5546 CNRS/University of Toulouse, 31326 Castanet-Tolosan, France

Arbuscular mycorrhizal (AM) fungi are obligate biotrophs that participate in a highly beneficial root symbiosis with 80% of land plants. Strigolactones are trace molecules in plant root exudates that are perceived by AM fungi at subnanomolar concentrations. Within just a few hours, they were shown to stimulate fungal mitochondria, spore germination, and branching of germinating hyphae. In this study we show that treatment of Gigaspora rosea with a strigolactone analog (GR24) causes a rapid increase in the NADH concentration, the NADH dehydrogenase activity, and the ATP content of the fungal cell. This fully and rapidly (within minutes) activated oxidative metabolism does not require new gene expression. Up-regulation of the genes involved in mitochondrial metabolism and hyphal growth, and stimulation of the fungal mitotic activity, take place several days after this initial boost to the cellular energy of the fungus. Such a rapid and powerful action of GR24 on G. rosea cells suggests that strigolactones are important plant signals involved in switching AM fungi toward full germination and a presymbiotic state.

^[C] Some figures in this article are displayed in color online but in black and white in print.

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.121400

^* Corresponding author; e-mail becard{at}scsv.ups-tlse.fr.

Received April 17, 2008; accepted June 25, 2008; published July 9, 2008.

This article has been cited by other articles:

				G. E. D. Oldroyd, M. J. Harrison, and U. Paszkowski Reprogramming Plant Cells for Endosymbiosis Science, May 8, 2009; 324(5928): 753 - 754. [Abstract] [Full Text] [PDF]