OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) OA All Versions of this Article: 148/3/1295    most recent pp.108.128256v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Osmani, S. A. Articles by Møller, B. L. Search for Related Content PubMed PubMed Citation Articles by Osmani, S. A. Articles by Møller, B. L. Agricola Articles by Osmani, S. A. Articles by Møller, B. L.

BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Catalytic Key Amino Acids and UDP-Sugar Donor Specificity of a Plant Glucuronosyltransferase, UGT94B1: Molecular Modeling Substantiated by Site-Specific Mutagenesis and Biochemical Analyses^1,[C],[OA]

VKR Research Centre Pro-Active Plants, DK–1871 Frederiksberg C, Copenhagen, Denmark (S.A.O., S.B., C.E.O., B.L.M.); Plant Biochemistry Laboratory, Department of Plant Biology and Biotechnology (S.A.O., S.B., B.L.M.), and Department of Natural Sciences (C.E.O.), University of Copenhagen, DK–1871 Frederiksberg C, Copenhagen, Denmark; and Centre de Recherches sur les Macromolécules Végétales, CNRS, 38041 Grenoble cedex 09, France (A.I.)

The plant UDP-dependent glucosyltransferase (UGT) BpUGT94B1 catalyzes the synthesis of a glucuronosylated cyanidin-derived flavonoid in red daisy (Bellis perennis). The functional properties of BpUGT94B1 were investigated using protein modeling, site-directed mutagenesis, and analysis of the substrate specificity of isolated wild-type and mutated forms of BpUGT94B1. A single unique arginine residue (R25) positioned outside the conserved plant secondary product glycosyltransferase region was identified as crucial for the activity with UDP-glucuronic acid. The mutants R25S, R25G, and R25K all exhibited only 0.5% to 2.5% of wild-type activity with UDP-glucuronic acid, but showed a 3-fold increase in activity with UDP-glucose. The model of BpUGT94B1 also enabled identification of key residues in the acceptor pocket. The mutations N123A and D152A decreased the activity with cyanidin 3-O-glucoside to less than 15% of wild type. The wild-type enzyme activity toward delphinidin-3-O-glucoside was only 5% to 10% of the activity with cyanidin 3-O-glucoside. Independent point mutations of three residues positioned near the acceptor B ring were introduced to increase the activity toward delphinidin-3-O-glucoside. In all three mutant enzymes, the enzymatic activity toward both acceptors was reduced to less than 15% of wild type. The model of BpUGT94B1 allowed for correct identification of catalytically important residues, within as well as outside the plant secondary product glycosyltransferase motif, determining sugar donor and acceptor specificity.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Birger Lindberg Møller (blm{at}life.ku.dk).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.128256

^* Corresponding author; e-mail blm{at}life.ku.dk.

Received August 20, 2008; accepted September 19, 2008; published October 1, 2008.

This article has been cited by other articles:

				S. Masada, K. Terasaka, Y. Oguchi, S. Okazaki, T. Mizushima, and H. Mizukami Functional and Structural Characterization of a Flavonoid Glucoside 1,6-Glucosyltransferase from Catharanthus roseus Plant Cell Physiol., August 1, 2009; 50(8): 1401 - 1415. [Abstract] [Full Text] [PDF]

				A. Noguchi, M. Horikawa, Y. Fukui, M. Fukuchi-Mizutani, A. Iuchi-Okada, M. Ishiguro, Y. Kiso, T. Nakayama, and E. Ono Local Differentiation of Sugar Donor Specificity of Flavonoid Glycosyltransferase in Lamiales PLANT CELL, May 1, 2009; 21(5): 1556 - 1572. [Abstract] [Full Text] [PDF]

				A. Rahier, M. Bergdoll, G. Genot, F. Bouvier, and B. Camara Homology Modeling and Site-Directed Mutagenesis Reveal Catalytic Key Amino Acids of 3{beta}-Hydroxysteroid-Dehydrogenase/C4-Decarboxylase from Arabidopsis Plant Physiology, April 1, 2009; 149(4): 1872 - 1886. [Abstract] [Full Text] [PDF]