This Article Full Text Full Text (PDF) All Versions of this Article: 148/3/1655    most recent pp.108.128769v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Rius, S. P. Articles by Gomez-Casati, D. F. Search for Related Content PubMed PubMed Citation Articles by Rius, S. P. Articles by Gomez-Casati, D. F. Agricola Articles by Rius, S. P. Articles by Gomez-Casati, D. F.

BIOENERGETICS AND PHOTOSYNTHESIS

Characterization of Arabidopsis Lines Deficient in GAPC-1, a Cytosolic NAD-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase^1,[C]

Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, CONICET/UNSAM, 7130, Chascomús, Argentina (S.P.R., D.F.G.-C.); Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, 2000, Rosario, Argentina (P.C.); and Laboratorio de Enzimología Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000, Santa Fe, Argentina (A.A.I.)

Phosphorylating glyceraldehyde-3-P dehydrogenase (GAPC-1) is a highly conserved cytosolic enzyme that catalyzes the conversion of glyceraldehyde-3-P to 1,3-bis-phosphoglycerate; besides its participation in glycolysis, it is thought to be involved in additional cellular functions. To reach an integrative view on the many roles played by this enzyme, we characterized a homozygous gapc-1 null mutant and an as-GAPC1 line of Arabidopsis (Arabidopsis thaliana). Both mutant plant lines show a delay in growth, morphological alterations in siliques, and low seed number. Embryo development was altered, showing abortions and empty embryonic sacs in basal and apical siliques, respectively. The gapc-1 line shows a decrease in ATP levels and reduced respiratory rate. Furthermore, both lines exhibit a decrease in the expression and activity of aconitase and succinate dehydrogenase and reduced levels of pyruvate and several Krebs cycle intermediates, as well as increased reactive oxygen species levels. Transcriptome analysis of the gapc-1 mutants unveils a differential accumulation of transcripts encoding for enzymes involved in carbon partitioning. According to these studies, some enzymes involved in carbon flux decreased (phosphoenolpyruvate carboxylase, NAD-malic enzyme, glucose-6-P dehydrogenase) or increased (NAD-malate dehydrogenase) their activities compared to the wild-type line. Taken together, our data indicate that a deficiency in the cytosolic GAPC activity results in modifications of carbon flux and mitochondrial dysfunction, leading to an alteration of plant and embryo development with decreased number of seeds, indicating that GAPC-1 is essential for normal fertility in Arabidopsis plants.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors is: Diego F. Gomez-Casati (diego.gomezcasati{at}intech.gov.ar).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.128769

^* Corresponding author; e-mail diego.gomezcasati{at}intech.gov.ar.

Received August 30, 2008; accepted September 22, 2008; published September 26, 2008.

This article has been cited by other articles:

				N. L. Houston, M. Hajduch, and J. J. Thelen Quantitative Proteomics of Seed Filling in Castor: Comparison with Soybean and Rapeseed Reveals Differences between Photosynthetic and Nonphotosynthetic Seed Metabolism Plant Physiology, October 1, 2009; 151(2): 857 - 868. [Abstract] [Full Text] [PDF]

				J. Munoz-Bertomeu, B. Cascales-Minana, J. M. Mulet, E. Baroja-Fernandez, J. Pozueta-Romero, J. M. Kuhn, J. Segura, and R. Ros Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Deficiency Leads to Altered Root Development and Affects the Sugar and Amino Acid Balance in Arabidopsis Plant Physiology, October 1, 2009; 151(2): 541 - 558. [Abstract] [Full Text] [PDF]