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BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function^{1,[C],[W],[OA]}

Laboratori del Suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, E–17071 Girona, Spain (O.S., M.S., M.M., M.F.); Institute of Cellular and Molecular Botany, University of Bonn, D–53115 Bonn, Germany (C.H., R.F., L.S.); CNRS, Université Louis Pasteur, Institut de Biologie Moléculaire des Plantes, F–67083 Strasbourg, France (V.S., F.P.); and Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma de Madrid, E–28049 Madrid, Spain (S.P.)

Suberin is a cell wall lipid polyester found in the cork cells of the periderm offering protection against dehydration and pathogens. Its biosynthesis and assembly, as well as its contribution to the sealing properties of the periderm, are still poorly understood. Here, we report on the isolation of the coding sequence CYP86A33 and the molecular and physiological function of this gene in potato (Solanum tuberosum) tuber periderm. CYP86A33 was down-regulated in potato plants by RNA interference-mediated silencing. Periderm from CYP86A33-silenced plants revealed a 60% decrease in its aliphatic suberin load and greatly reduced levels of C18:1 -hydroxyacid (approximately 70%) and ,-diacid (approximately 90%) monomers in comparison with wild type. Moreover, the glycerol esterified to suberin was reduced by 60% in the silenced plants. The typical regular ultrastructure of suberin, consisting of dark and light lamellae, disappeared and the thickness of the suberin layer was clearly reduced. In addition, the water permeability of the periderm isolated from CYP86A33-silenced lines was 3.5 times higher than that of the wild type. Thus, our data provide convincing evidence for the involvement of -functional fatty acids in establishing suberin structure and function.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Mercè Figueras (merce.figueras{at}udg.edu).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

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www.plantphysiol.org/cgi/doi/10.1104/pp.108.127183

^* Corresponding author; e-mail merce.figueras{at}udg.edu.

Received July 30, 2008; accepted December 18, 2008; published December 24, 2008.

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