This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 149/2/1111    most recent pp.108.127514v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Chen, T. Articles by Lin, J. Search for Related Content PubMed PubMed Citation Articles by Chen, T. Articles by Lin, J. Agricola Articles by Chen, T. Articles by Lin, J.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Combined Proteomic and Cytological Analysis of Ca²⁺-Calmodulin Regulation in Picea meyeri Pollen Tube Growth^1,[C],[W]

Tong Chen, Xiaoqin Wu, Yanmei Chen, Xiaojuan Li, Mei Huang, Maozhong Zheng, Frantiek Baluka, Jozef amaj and Jinxing Lin^*

Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (T.C., X.W., Y.C., X.L., M.H., M.Z., J.L.); Systematic and Evolutionary Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (X.W.); Graduate School of Chinese Academy of Sciences, Beijing 100039, China (M.Z.); Institute of Cellular and Molecular Botany, University of Bonn, D–53115 Bonn, Germany (F.B., J..); Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, SK–95007 Nitra, Slovak Republic (J..); and Faculty of Natural Sciences, Palack University Olomouc, 771 47 Olomouc, Czech Republic (J..)

Ca²⁺-calmodulin (Ca²⁺-CaM) is a critical molecule that mediates cellular functions by interacting with various metabolic and signaling pathways. However, the protein expression patterns and accompanying serial cytological responses in Ca²⁺-CaM signaling deficiency remain enigmatic. Here, we provide a global analysis of the cytological responses and significant alterations in protein expression profiles after trifluoperazine treatment in Picea meyeri, which abrogates Ca²⁺-CaM signaling. Ninety-three differentially displayed proteins were identified by comparative proteomics at different development stages and were assigned to different functional categories closely related to tip growth machinery. The inhibition of Ca²⁺-CaM signaling rapidly induced an increase in extracellular Ca²⁺ influx, resulting in dramatically increased cytosolic Ca²⁺ concentrations and ultrastructural abnormalities in organelles as the primary responses. Secondary and tertiary alterations included actin filament depolymerization, disrupted patterns of endocytosis and exocytosis, and cell wall remodeling, ultimately resulting in perturbed pollen tube extension. In parallel with these cytological events, time-course experiments revealed that most differentially expressed proteins showed time-dependent quantitative changes (i.e. some signaling proteins and proteins involved in organelle functions and energy production changed first, followed by alterations in proteins related to cytoskeletal organization, secretory pathways, and polysaccharide synthesis). Taken together, Ca²⁺-CaM dysfunction induced serial cytological responses and temporal changes in protein expression profiles, indicating the pivotal role of Ca²⁺-CaM in the regulation of tip growth machinery.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Jinxing Lin (linjx{at}ibcas.ac.cn).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.127514

^* Corresponding author; e-mail linjx{at}ibcas.ac.cn.

Received August 2, 2008; accepted November 11, 2008; published November 14, 2008.