OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 149/2/1196    most recent pp.108.131508v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Schmitz, R. J. Articles by Amasino, R. M. Search for Related Content PubMed PubMed Citation Articles by Schmitz, R. J. Articles by Amasino, R. M. Agricola Articles by Schmitz, R. J. Articles by Amasino, R. M.

SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION

Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis^{1,[C],[W],[OA]}

Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706 (R.J.S., Y.T., M.R.D., R.M.A.); and Department of Plant Biology, University of Georgia, Athens, Georgia 30602 (X.Z.)

The spectrum of histone modifications at a given locus is a critical determinant for the correct output of gene expression. In Arabidopsis (Arabidopsis thaliana), many studies have examined the relationship between histone methylation and gene expression, but few studies exist on the relationship between other covalent histone modifications and gene expression. In this work, we describe the role of histone H2B deubiquitination in the activation of gene expression and the consequence of a perturbation of histone H2B deubiquitination in the timing of the floral transition in Arabidopsis. A mutation in a H2B deubiquitinase, UBIQUITIN-SPECIFIC PROTEASE26 (UBP26), results in an early-flowering phenotype. In the ubp26 mutant, mRNA levels of the floral repressor FLOWERING LOCUS C (FLC) and other related family members is decreased. Furthermore, this mutant accumulates H2B monoubiquitination, and has decreased levels of H3K36 trimethylation and increased levels of H3K27 trimethylation at the FLC locus. Thus, UBP26 is required for transcriptional activation of FLC through H2B deubiquitination and is consistent with a model in which deubiquitination is necessary for the accumulation of H3K36 trimethylation and the proper level of transcriptional activation.

² These authors contributed equally to the article.

³ Present address: Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037.

The author responsible for the distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Richard Mark Amasino (amasino{at}biochem.wisc.edu).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[W] The online version of this article contains Web-only data.

^[OA] Open access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.131508

^* Corresponding author; e-mail amasino{at}biochem.wisc.edu.

Received November 5, 2008; accepted December 7, 2008; published December 17, 2008.

This article has been cited by other articles:

				Y. Tamada, J.-Y. Yun, S. c. Woo, and R. M. Amasino ARABIDOPSIS TRITHORAX-RELATED7 Is Required for Methylation of Lysine 4 of Histone H3 and for Transcriptional Activation of FLOWERING LOCUS C PLANT CELL, October 1, 2009; 21(10): 3257 - 3269. [Abstract] [Full Text] [PDF]