OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 150/1/308    most recent pp.108.131979v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Plant Physiol. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yamaguchi, T. Articles by Shibuya, N. Search for Related Content PubMed PubMed Citation Articles by Yamaguchi, T. Articles by Shibuya, N. Agricola Articles by Yamaguchi, T. Articles by Shibuya, N.

PLANTS INTERACTING WITH OTHER ORGANISMS

Suppression of a Phospholipase D Gene, OsPLDβ1, Activates Defense Responses and Increases Disease Resistance in Rice^{1,[C],[W],[OA]}

National Agricultural Research Center, Joetsu, Niigata 943–0193, Japan (T.Y., M.K., H.Y., T.A., K.H.); and Department of Life Sciences, Faculty of Agriculture, Meiji University, Kawasaki, Kanagawa 214–8571, Japan (L.K., T.S., N.S.)

Phospholipase D (PLD) plays an important role in plants, including responses to abiotic as well as biotic stresses. A survey of the rice (Oryza sativa) genome database indicated the presence of 17 PLD genes in the genome, among which OsPLD1, OsPLD5, and OsPLDβ1 were highly expressed in most tissues studied. To examine the physiological function of PLD in rice, we made knockdown plants for each PLD isoform by introducing gene-specific RNA interference constructs. One of them, OsPLDβ1-knockdown plants, showed the accumulation of reactive oxygen species in the absence of pathogen infection. Reverse transcription-polymerase chain reaction and DNA microarray analyses revealed that the knockdown of OsPLDβ1 resulted in the up-/down-regulation of more than 1,400 genes, including the induction of defense-related genes such as pathogenesis-related protein genes and WRKY/ERF family transcription factor genes. Hypersensitive response-like cell death and phytoalexin production were also observed at a later phase of growth in the OsPLDβ1-knockdown plants. These results indicated that the OsPLDβ1-knockdown plants spontaneously activated the defense responses in the absence of pathogen infection. Furthermore, the OsPLDβ1-knockdown plants exhibited increased resistance to the infection of major pathogens of rice, Pyricularia grisea and Xanthomonas oryzae pv oryzae. These results suggested that OsPLDβ1 functions as a negative regulator of defense responses and disease resistance in rice.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Takeshi Yamaguchi (tkyama{at}affrc.go.jp).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.131979

^* Corresponding author; e-mail tkyama{at}affrc.go.jp.

Received November 25, 2008; accepted March 11, 2009; published March 13, 2009.

Related articles in Plant Physiol.:

On the Inside

Peter V. Minorsky
Plant Physiol. 2009 150: 1-2. [Full Text]