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PLANTS INTERACTING WITH OTHER ORGANISMS

Genetic Dissection of Verticillium Wilt Resistance Mediated by Tomato Ve1^{1,[C],[W],[OA]}

Laboratory of Phytopathology, Wageningen University, 6709 PD Wageningen, The Netherlands (E.F.F., Z.Z., B.P.H.J.T.); Centre for BioSystems Genomics, 6700 AB Wageningen, The Netherlands (E.F.F., B.P.H.J.T.); Center for Signal Transduction and Metabolomics, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (Z.Z., C.-M.L.); and Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1 (J.C.J.A., C.D.M.C., R.N.N., J.R.)

Vascular wilt diseases caused by soil-borne pathogens are among the most devastating plant diseases worldwide. The Verticillium genus includes vascular wilt pathogens with a wide host range. Although V. longisporum infects various hosts belonging to the Cruciferaceae, V. dahliae and V. albo-atrum cause vascular wilt diseases in over 200 dicotyledonous species, including economically important crops. A locus responsible for resistance against race 1 strains of V. dahliae and V. albo-atrum has been cloned from tomato (Solanum lycopersicum) only. This locus, known as Ve, comprises two closely linked inversely oriented genes, Ve1 and Ve2, that encode cell surface receptor proteins of the extracellular leucine-rich repeat receptor-like protein class of disease resistance proteins. Here, we show that Ve1, but not Ve2, provides resistance in tomato against race 1 strains of V. dahliae and V. albo-atrum and not against race 2 strains. Using virus-induced gene silencing in tomato, the signaling cascade downstream of Ve1 is shown to require both EDS1 and NDR1. In addition, NRC1, ACIF, MEK2, and SERK3/BAK1 also act as positive regulators of Ve1 in tomato. In conclusion, Ve1-mediated resistance signaling only partially overlaps with signaling mediated by Cf proteins, type members of the receptor-like protein class of resistance proteins.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Bart P. H. J. Thomma (bart.thomma{at}wur.nl).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[W] The online version of this article contains Web-only data.

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www.plantphysiol.org/cgi/doi/10.1104/pp.109.136762

^* Corresponding author; e-mail bart.thomma{at}wur.nl.

Received February 6, 2009; accepted March 17, 2009; published March 25, 2009.

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