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ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Disruption of OsYSL15 Leads to Iron Inefficiency in Rice Plants^{1,[C],[W],[OA]}

Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology, Pohang 790–784, Republic of Korea (S.L., Y.L., G.A.); Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003 (J.C.C., E.L.W.); and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755 (S.A.K., M.L.G.)

Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe³⁺; these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion to β-glucuronidase showed that it was expressed in all root tissues when Fe was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15:green fluorescent protein fusion was localized to the plasma membrane. OsYSL15 functionally complemented yeast strains defective in Fe uptake on media containing Fe³⁺-deoxymugineic acid and Fe²⁺-nicotianamine. Two insertional osysl15 mutants exhibited chlorotic phenotypes under Fe deficiency and had reduced Fe concentrations in their shoots, roots, and seeds. Nitric oxide treatment reversed this chlorosis under Fe-limiting conditions. Overexpression of OsYSL15 increased the Fe concentration in leaves and seeds from transgenic plants. Altogether, these results demonstrate roles for OsYSL15 in Fe uptake and distribution in rice plants.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Gynheung An (genean{at}postech.ac.kr).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[W] The online version of this article contains Web-only data.

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www.plantphysiol.org/cgi/doi/10.1104/pp.109.135418

^* Corresponding author; e-mail genean{at}postech.ac.kr.

Received January 8, 2009; accepted April 14, 2009; published April 17, 2009.