OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 150/3/1260    most recent pp.109.136812v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Cai, W. Articles by Zhang, L. Search for Related Content PubMed PubMed Citation Articles by Cai, W. Articles by Zhang, L. Agricola Articles by Cai, W. Articles by Zhang, L.

BIOENERGETICS AND PHOTOSYNTHESIS

LPA66 Is Required for Editing psbF Chloroplast Transcripts in Arabidopsis^1,[W],[OA]

Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (W.C., D.J., L.P., J.G., J.M., M.Z., C.L., L.Z.); and School of Life Sciences, Lanzhou University, Lanzhou 73000, China (W.C.)

To gain insight into the molecular mechanism of RNA editing, we have characterized the low psii accumulation66 (lpa66) Arabidopsis (Arabidopsis thaliana) mutant, which displays a high chlorophyll fluorescence phenotype. Its perturbed chlorophyll fluorescence is reflected in reduced levels of photosystem II (PSII) proteins. In vivo protein labeling showed that synthesis rates of the PSII reaction center protein D1/D2 were lower, and turnover rates of PSII core proteins higher, than in wild-type counterparts. The assembly of newly synthesized proteins into PSII occurs in the lpa66 mutant but with reduced efficiency compared with the wild type. LPA66 encodes a chloroplast protein of the pentatricopeptide repeat family. In lpa66 mutants, editing of psbF that converts serine to phenylalanine is specifically impaired. Thus, LPA66 is specifically required for editing the psbF transcripts in Arabidopsis, and the amino acid alternation due to lack of editing strongly affects the efficiency of the assembly of PSII complexes.

² These authors contributed equally to the article.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Lixin Zhang (zhanglixin{at}ibcas.ac.cn).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.109.136812

^* Corresponding author; e-mail zhanglixin{at}ibcas.ac.cn.

Received February 10, 2009; accepted May 11, 2009; published May 15, 2009.

This article has been cited by other articles:

				K. Yura, S. Sulaiman, Y. Hatta, M. Shionyu, and M. Go RESOPS: A Database for Analyzing the Correspondence of RNA Editing Sites to Protein Three-Dimensional Structures Plant Cell Physiol., November 1, 2009; 50(11): 1865 - 1873. [Abstract] [Full Text] [PDF]

				K. Hammani, K. Okuda, S. K. Tanz, A.-L. Chateigner-Boutin, T. Shikanai, and I. Small A Study of New Arabidopsis Chloroplast RNA Editing Mutants Reveals General Features of Editing Factors and Their Target Sites PLANT CELL, November 1, 2009; 21(11): 3686 - 3699. [Abstract] [Full Text] [PDF]