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SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION

CDKB1;1 Forms a Functional Complex with CYCA2;3 to Suppress Endocycle Onset^1,[W],[OA]

Department of Plant Systems Biology, Flanders Institute for Biotechnology, 9052 Ghent, Belgium (V.B., T.L., J.B., J.V.L., H.V.D.D., S.M., G.V.I., E.R., G.D.J., D.I., L.D.V.); Department of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium (V.B., T.L., J.B., J.V.L., H.V.D.D., S.M., G.V.I., E.R., G.D.J., D.I., L.D.V.); Institut des Sciences du Végétal, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2355, 91198 Gif-sur-Yvette, France (E.K.); Institute for Plant Genomics, Human Biotechnology, and Bioenergy, Bay Zoltan Foundation for Applied Research, 6726 Szeged, Hungary (E.K.); Department of Biology, Center for Proteome Analysis and Mass Spectrometry, University of Antwerp, 2020 Antwerp, Belgium (E.W.); and Flemish Institute for Technological Research, 2400 Mol, Belgium (E.W.)

The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 was identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 copurifies and associates with the A2-type cyclin CYCA2;3. Coexpression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Lieven De Veylder (lieven.deveylder{at}psb.vib-ugent.be).

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www.plantphysiol.org/cgi/doi/10.1104/pp.109.140269

^* Corresponding author; e-mail lieven.deveylder{at}psb.vib-ugent.be.

Received April 22, 2009; accepted May 15, 2009; published May 20, 2009.

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