OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) OA All Versions of this Article: 150/3/1567    most recent pp.109.138131v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leiss, K. A. Articles by Klinkhamer, P. G.L. Search for Related Content PubMed PubMed Citation Articles by Leiss, K. A. Articles by Klinkhamer, P. G.L. Agricola Articles by Leiss, K. A. Articles by Klinkhamer, P. G.L.

PLANTS INTERACTING WITH OTHER ORGANISMS

Identification of Chlorogenic Acid as a Resistance Factor for Thrips in Chrysanthemum^[C],[OA]

Research Group Plant Ecology, Institute of Biology, Leiden University, 2311 GP Leiden, The Netherlands (K.A.L., P.G.L.K.); and Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands (F.M., Y.H.C., R.V.)

Western flower thrips (Frankliniella occidentalis) has become a key insect pest of agricultural and horticultural crops worldwide. Little is known about host plant resistance to thrips. In this study, we investigated thrips resistance in chrysanthemum (Dendranthema grandiflora). We identified thrips-resistant chrysanthemums applying bioassays. Subsequently, nuclear magnetic resonance (NMR)-based metabolomics was applied to compare the metabolome of thrips-resistant and -susceptible chrysanthemums. NMR facilitates wide-range coverage of the metabolome. We show that thrips-resistant and -susceptible chrysanthemums can be discriminated on basis of their metabolomic profiles. Thrips-resistant chrysanthemums contained higher amounts of the phenylpropanoids chlorogenic acid and feruloyl quinic acid. Both phenylpropanoids are known for their inhibitory effect on herbivores as well as pathogens. Thus, chlorogenic and feruloyl quinic acid are the compounds of choice to improve host plants resistance to thrips in ornamentals and crops. The effect of chlorogenic acid on thrips was further studied in bioassays with artificial diets. These experiments confirmed the negative effects on thrips. Our results prove NMR to be an important tool to identify different metabolites involved in herbivore resistance. It constitutes a significant advance in the study of plant-insect relationships, providing key information on the implementation of herbivore resistance breeding strategies in plants.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Kirsten A. Leiss (k.a.leiss{at}biology.leidenuniv.nl).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[OA] Open access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.109.138131

^* Corresponding author; e-mail k.a.leiss{at}biology.leidenuniv.nl.

Received March 5, 2009; accepted May 8, 2009; published May 15, 2009.

This article has been cited by other articles:

				P. V. Demkura, G. Abdala, I. T. Baldwin, and C. L. Ballare Jasmonate-Dependent and -Independent Pathways Mediate Specific Effects of Solar Ultraviolet B Radiation on Leaf Phenolics and Antiherbivore Defense Plant Physiology, February 1, 2010; 152(2): 1084 - 1095. [Abstract] [Full Text] [PDF]

				A. D. Hegeman Plant metabolomics--meeting the analytical challenges of comprehensive metabolite analysis Briefings in Functional Genomics, January 11, 2010; (2010) elp053v1. [Abstract] [Full Text] [PDF]