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GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Transcriptional Gene Silencing Mediated by a Plastid Inner Envelope Phosphoenolpyruvate/Phosphate Translocator CUE1 in Arabidopsis^1,[OA]

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (J.S., X.R., R.C., J.L., Z.G.); China Agricultural University-University of California Riverside Center for Biological Sciences and Biotechnology, Beijing 100193, China (Z.G.); and National Center for Plant Gene Research, Beijing 100193, China (Z.G.)

Mutations in REPRESSOR OF SILENCING1 (ROS1) lead to the transcriptional gene silencing (TGS) of Pro_RD29A:LUC (LUCIFERASE) and Pro_35S:NPTII (Neomycin Phosphotransferase II) reporter genes. We performed a genetic screen to find suppressors of ros1 that identified two mutant alleles in the Arabidopsis (Arabidopsis thaliana) CHLOROPHYLL A/B BINDING PROTEIN UNDEREXPRESSED1 (CUE1) gene, which encodes a plastid inner envelope phosphoenolpyruvate/phosphate translocator. The cue1 mutations released the TGS of Pro_35S:NPTII and the transcriptionally silent endogenous locus TRANSCRIPTIONAL SILENCING INFORMATION in a manner that was independent of DNA methylation but dependent on chromatin modification. The cue1 mutations did not affect the TGS of Pro_RD29A:LUC in ros1, which was dependent on RNA-directed DNA methylation. It is possible that signals from chloroplasts help to regulate the epigenetic status of a subset of genomic loci in the nucleus.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Zhizhong Gong (gongzz{at}cau.edu.cn).

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www.plantphysiol.org/cgi/doi/10.1104/pp.109.139626

^* Corresponding author; e-mail gongzz{at}cau.edu.cn.

Received April 13, 2009; accepted June 4, 2009; published June 10, 2009.