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ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Physiological and Transcriptome Analysis of Iron and Phosphorus Interaction in Rice Seedlings^1,[C],[W]

Joint Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (L.Z., F. Huang, R.N., J.W., E.G., F. He, L.C., F.W., P.W., J.W., H.S.); State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (L.Z., F. Huang, J.W., L.C., F.W., P.W., H.S.); Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia 6009, Australia (R.N., E.G., J.W.); and Shanghai Biochip Company, Shanghai 201203, China (F. He)

The antagonistic interaction between iron (Fe) and phosphorus (P) has been noted in the area of plant nutrition. To understand the physiology and molecular mechanisms of this interaction, we studied the growth performance, nutrient concentration, and gene expression profiles of root and shoot segments derived from 10-d-old rice (Oryza sativa) seedlings under four different nutrient conditions: (1) full strength of Fe and P (+Fe+P); (2) full strength of P and no Fe (–Fe+P); (3) full strength of Fe and no P (+Fe–P); and (4) without both Fe and P (–Fe–P). While removal of Fe in the growth medium resulted in very low shoot and root Fe concentrations, the chlorotic symptoms and retarded seedling growth were only observed on seedlings grown in the presence of P. Microarray data showed that in roots, 7,628 transcripts were significantly changed in abundance in the absence of Fe alone. Interestingly, many of these changes were reversed if P was also absent (–Fe–P), with only approximately 15% overlapping with –Fe alone (–Fe+P). Analysis of the soluble Fe concentration in rice seedling shoots showed that P deficiency resulted in significantly increased Fe availability within the plants. The soluble Fe concentration under –Fe–P conditions was similar to that under +Fe+P conditions. These results provide evidence that the presence of P can affect Fe availability and in turn can influence the regulation of Fe-responsive genes.

² These authors contributed equally to the article.

³ Present address: College of Forestry and Biotechnology, Zhejiang Forestry University, Linan 311300, China.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Huixia Shou (huixia{at}zju.edu.cn).

^[C] Some figures in this article are displayed in color online but in black and white in the print edition.

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.109.141051

^* Corresponding author; e-mail huixia{at}zju.edu.cn.

Received May 8, 2009; accepted July 8, 2009; published July 15, 2009.

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