Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online August 5, 2009; 10.1104/pp.109.142133

Plant Physiology 151:732-740 (2009)
© 2009 American Society of Plant Biologists

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow OA Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
151/2/732    most recent
pp.109.142133v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Steffens, B.
Right arrow Articles by Sauter, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steffens, B.
Right arrow Articles by Sauter, M.
Agricola
Right arrow Articles by Steffens, B.
Right arrow Articles by Sauter, M.
Related Collections
Right arrow The Grasses
ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Heterotrimeric G Protein Signaling Is Required for Epidermal Cell Death in Rice[W],[OA]

Bianka Steffens and Margret Sauter*

Physiologie und Entwicklungsbiologie der Pflanzen, Botanisches Institut, Universität Kiel, 24118 Kiel, Germany

In rice (Oryza sativa) adventitious root primordia are formed at the nodes as part of normal development. Upon submergence of rice plants, adventitious roots emerge from the nodes preceded by death of epidermal cells above the root primordia. Cell death is induced by ethylene and mediated by hydrogen peroxide (H2O2). Pharmacological experiments indicated that epidermal cell death was dependent on signaling through G proteins. Treatment with GTP-{gamma}-S induced epidermal cell death, whereas GDP-β-S partially inhibited ethylene-induced cell death. The dwarf1 (d1) mutant of rice has repressed expression of the G{alpha} subunit RGA1 of heterotrimeric G protein. In d1 plants, cell death in response to ethylene and H2O2 was nearly completely abolished, indicating that signaling through G{alpha} is essential. Ethylene and H2O2 were previously shown to alter gene expression in epidermal cells that undergo cell death. Transcriptional regulation was not generally affected in the d1 mutant, indicating that altered gene expression is not sufficient to trigger cell death in the absence of G{alpha}. Analysis of genes encoding proteins related to G protein signaling revealed that four small GTPase genes, two GTPase-activating protein genes, and one GDP dissociation inhibitor gene but not RGA1 were differentially expressed in epidermal cells above adventitious roots, indicating that G{alpha} activity is regulated posttranscriptionally.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Margret Sauter (msauter{at}bot.uni-kiel.de).

[W] The online version of this article contains Web-only data.

[OA] Open access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.109.142133

* Corresponding author; e-mail msauter{at}bot.uni-kiel.de.

Received May 27, 2009; accepted July 31, 2009; published August 5, 2009.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2009 by the American Society of Plant Biologists