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First published online August 19, 2009; 10.1104/pp.109.141390

Plant Physiology 151:882-892 (2009)
© 2009 American Society of Plant Biologists

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BIOENERGETICS AND PHOTOSYNTHESIS

An Rrf2-Type Transcriptional Regulator Is Required for Expression of psaAB Genes in the Cyanobacterium Synechocystis sp. PCC 68031,[W],[OA]

Takafumi Midorikawa, Koji Matsumoto, Rei Narikawa and Masahiko Ikeuchi*

Department of Biological Science, Graduate School of Sciences (T.M., K.M., M.I.), and Department of Life Sciences (Biology), Graduate School of Arts and Sciences (R.N., M.I.), University of Tokyo, Tokyo 153–8902, Japan

Photosynthetic organisms must regulate photosystem stoichiometry (photosystem I-to-photosystem II ratio) under various light conditions. Transcriptional regulation of the psaAB genes is a critical process for this photoacclimation in cyanobacteria. In the course of our screening of transcriptional regulators in the cyanobacterium Synechocystis sp. PCC 6803, we found that chlorophyll accumulation was impaired in an Rrf2-type regulator Slr0846 mutant. DNA microarray and primer extension analyses showed that the expression of psaAB genes was markedly decreased in the mutant. Consistently, the mutant exhibited lower photosystem I-to-photosystem II ratio under normal light conditions, suggestive of decreased accumulation of the photosystem I reaction center. Gel-shift assay confirmed that the Slr0846 protein bound to a far upstream promoter region of psaAB. These phenotypes of the mutant varied substantially with light conditions. These results suggest that Slr0846 is a novel transcriptional regulator for optimal expression of psaAB.

1 This work was supported by Grants-in-Aid for Scientific Research (to R.N. and M.I.) and the Global Center-of-Excellence Program (Integrative Life Science Based on the Study of Biosignaling Mechanisms) (to T.M.) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Masahiko Ikeuchi (mikeuchi{at}bio.c.u-tokyo.ac.jp).

[W] The online version of this article contains Web-only data.

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www.plantphysiol.org/cgi/doi/10.1104/pp.109.141390

* Corresponding author; e-mail mikeuchi{at}bio.c.u-tokyo.ac.jp.

Received May 13, 2009; accepted August 14, 2009; published August 19, 2009.

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