Plant Physiol. Bio-Rad Microplate Reader
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Plant Physiology 42:113-119 (1967)
© 1967 American Society of Plant Biologists

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Barley Endosperm Bioassay for Gibberellins. II. Application of the Method

B. G. Coombe, D. Cohen1 and L. G. Paleg

Department of Plant Physiology, Waite Agricultural Research Institute, The University of Adelaide, South Australia

The use of the modified endosperm bioassay in conjunction with techniques for extracting and paper chromatographing extracts, and the application of statistical analyses to the results, is reported. The modified procedure has not altered the relative response to different gibberellins, though an analysis of many standard curves indicates that at least 1 feature of the bioassay system is still uncontrolled. A comparison of this bioassay with 15 others indicates that the endosperm response may have wide applicability in explorations of the physiological effects of gibberellins.


1 Present address: Biology Department, Carleton University, Ottawa, Canada.




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B. G. Coombe
GA32: A Polar Gibberellin with High Biological Potency
Science, May 21, 1971; 172(3985): 856 - 857.
[Abstract] [PDF]




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Copyright © 1967 by the American Society of Plant Biologists