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Labeling Pattern of Translocated Stachyose in Squash ¹

Department of Botany, The Ohio State University, Columbus, Ohio 43210

One mature blade of each squash plant was continuously labeled with ¹⁴CO₂ for 15, 30, or 70 minutes in light. The ethanol soluble materials from serial sections of petioles were extracted and separated by paper chromatography. The ratios of label in the various components of this fraction were determined. Stachyose, which contained the major portion of the label of this fraction, was hydrolyzed and the resultant hydrolysate was separated by paper chromatography. Specific activities of the hexoses derived from stachyose were determined. It was found that the glucose and fructose moieties of stachyose became labeled at the same rates; however, the galactose moiety became labeled more rapidly.

¹ This investigation was supported in part by a National Science Foundation grant to Dr. C. A. Swanson (G-24040). These data are from a thesis presented to the Graduate School of The Ohio State University in partial fulfillment of the requirements for the doctorate degree. Publication No. 748 of the Graduate Faculty of Botany, The Ohio State University.