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Plant Physiology 43:1703-1709 (1968)
© 1968 American Society of Plant Biologists

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Articles

Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-D-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus 1

H. M. Flowers2, K. K. Batra, Jennifer Kemp and W. Z. Hassid

Department of Biochemistry, University of California, Berkeley, California 94720

Particulate, and digitonin-solubilized, enzyme systems from Phaseolus aureus and Lupinus albus catalyze the biosynthesis of aqueous-insoluble glucans from UDP-D-glucose. The digitonin treatment greatly increases the enzymic activity of (per unit protein) both the 34,000g pellet and the supernatant liquid as compared with that of the original particles. Most of the polymer produced (90-95%) is soluble in hot, dilute alkali; the interglucosidic linkages of the alkali-soluble and alkali-insoluble polymers are identical. The optimum concentration for the incorporation of radioactivity from UDP-D-glucose-14C into soluble glucan is high; at 10–3 M at least 50% of the added radioactive glucosyl donor is incorporated.

Careful examination of the products of degradation of the polymers produced by various enzymic preparations showed that {beta}-(1->3)-glucans are produced. No evidence was obtained for any measurable amount of {beta}-(1->4)-D-glucose linkages.

2 On leave of absence from Weizmann Institute of Science, Rehovoth, Israel.

1 This investigation was supported in part by a research grant A-1418 from the National Institutes of Health, United States Public Health Service and by a resarch grant G-23763 from the National Science Foundation. Support of this work by the Agricultural Experiment Station is also acknowledged.




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W. Z. Hassid
Biosynthesis of Oligosaccharides and Polysaccharides in Plants
Science, July 11, 1969; 165(3889): 137 - 144.
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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1968 by the American Society of Plant Biologists