This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Richter, M. Articles by Scheffer, F. Search for Related Content PubMed PubMed Citation Articles by Richter, M. Articles by Scheffer, F. Agricola Articles by Richter, M. Articles by Scheffer, F.

Articles

Determination of Root Exudates in a Steril Continuous Flow Culture. I. The Culture Method

Institut fuer Bodenkunde der Georg August Universitaet, Goettingen, Germany

A sterile plant culture consisting of culture vessels, culture solution container, collecting flasks for percolating nutrient solution, illumination and aeration systems and a suitable pump is described. Its difference with other culture methods is a very slow, continuous percolation of the nutrient solution through the rooting medium. Well defined and controllable conditions can thus be established in the rhizosphere over long culture periods. Samples can be collected at short intervals without disturbing the rhizosphere in any way nor endangering the sterility of the culture. One of the fundamental factors determining the special ecological characteristics of the plant rhizosphere is the liberation of organic and inorganic substances by the plant root. During the study of this phenomenon it became evident that the amount of substances liberated varies within wide limits (factors 100 to 1000) according to the conditions in which the root is developing.