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Plant Physiology 43:1859-1865 (1968)
© 1968 American Society of Plant Biologists

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Synthesis of Chymotrypsin Inhibitor I Protein in Potato Leaflets Induced by Detachment 1

C. A. Ryan2

Department of Agricultural Chemistry, Washington State University, Pullman, Washington 99163

Chymotrypsin inhibitor I is a protein that can be induced to accumulate in potato leaflets within hr in the light when leaflets are detached from intact plants and supplied with water. This increase in inhibitor I is not accompanied by an increase in all proteins in the detached leaflets. The accumulation is polar, proceeding from the base of the leaflets toward the tip. Low inhibitor I levels can be effectively maintained in detached leaflets by supplying them either water in the dark or solutions of indole acetic acid or 2,4-dichlorophenoxy acetic acid in the light. Inhibitor I from leaflets is not identical to inhibitor I isolated from potato tubers as determined by immunochemical analyses.


2 Research Career Development Awardee of the United States Public Health Service.

1 This work was supported by United States Public Health Service Grants 1-K3-Gm 17059 and GM 12505. College of Agriculture Scientific Paper #3161, Project #791.







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Copyright © 1968 by the American Society of Plant Biologists