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Plant Physiology 43:1906-1912 (1968)
© 1968 American Society of Plant Biologists

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Purification and Some Properties of the Nitrogenase From Soybean (Glycine max Merr.) Nodules 1

Robert V. Klucas2, Burton Koch, Sterling A. Russell and Harold J. Evans

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331

The nitrogenase system in cell-free extracts of soybean nodule bacteroids was fractionated into 2 components by use of protamine sulfate or polypropylene glycol precipitation followed by chromatography on DEAE-cellulose. Iron and molybdenum were concentrated in 1 fraction and iron in the other. Combination of fractions resulted in a striking stimulation in activity relative to the activity of individual fractions. The effect of different proportions of the 2 fractions on specific activities was studied. The ratios of the rates of reduction of acetylene and N2 by extracts or fractions of different purities were relatively constant. Extracts or fractions retained most of their nitrogenase activities when stored in liquid N2.

2 United States Public Health Service Fellow (GM 35-348-01).

1 This research was supported by NSF grant GB5185X and by the Oregon Agricultural Experiment Station (Technical Paper No. 2532).






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Copyright © 1968 by the American Society of Plant Biologists