This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Killion, D. D. Articles by Frans, R. E. Search for Related Content PubMed PubMed Citation Articles by Killion, D. D. Articles by Frans, R. E. Agricola Articles by Killion, D. D. Articles by Frans, R. E.

Articles

Oxidative and Phosphorylative Activities of Mitochondria Isolated From Cotton Hypocotyls ¹

Department of Agronomy, Agricultural Experiment Station, University of Arkansas, Fayetteville, Arkansas 72701

Mitochondria isolated from 2 strains of cotton plant hypocotyls (Gossypium hirsutum L. var. Rex smooth leaf and Rex glandless) were examined for their oxidative phosphorylation activities. Bovine serum albumin at a relatively high concentration was essential in the extraction medium for the isolation of oxidatively active mitochondria from both strains of cotton. Phosphorylation was obtained only with Rex glandless cotton mitochondria. This activity was low in comparison to the mitochondria isolated from soybeans (Glycine max L. var. Lee). The endogenous gossypol content was found to be much higher in the Rex smooth leaf tissue than in the Rex glandless tissue. In turn, comparable gossypol differences were found associated with their respective mitochondrial fractions. Exogenous gossypol uncoupled succinate oxidation with active mitochondria isolated from soybeans. Gossypol as a possible uncoupler is discussed and compared to carbonyl cyanide, m-chlorophenyl hydrazone and 2,4-dinitrophenol.