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Plant Physiology 43:1284-1290 (1968)
© 1968 American Society of Plant Biologists

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Isolation of Mutants of Euglena gracilis With Impaired Photosynthesis 1

George K. Russell2 and Harvard Lyman

Department of Biology, Princeton University, Princeton, New Jersey, Microbiology Division, Medical Research Center, Brookhaven National Laboratory, Upton, New York

Four mutant strains of Euglena gracilis have been isolated after treatment of wild type cells with ultraviolet light or the chemical mutagen nitrosoguanidine. None of the mutants is capable of autotrophic growth or photosynthetic carbon dioxide fixation.

The mutant strains contain normal amounts of the enzymes of the reductive pentose phosphate cycle and are qualitatively similar to the wild type in pigment composition, but are unable to carry out the Hill reaction (light induced reduction of 2,6-dichlorophenol indophenol). Isolated mutant plastids cannot photoreduce NADP with water as the electron donor but can carry out this reaction when the electron donating system is ascorbate and 2,6-dichlorophenol indophenol. Whole cells of the mutants show the light induced oxidation of cytochrome f by light reaction I but are unable to bring about cytochrome f reduction by light reaction II. The mutants appear to be blocked at or near light reaction II in the photosynthetic electron transport chain.

The mutants may represent alterations of the chloroplast genome since the mutation isolation was carried out under conditions where chloroplast viability was severely impaired, but cell viability was unaffected.

2 Present address: Department of Biology, Adelphi University, Garden City, New York 11530.

1 This investigation was aided by grants from the National Science Foundation (GB 4075) and the Eugene P. Higgins Fund of Princeton University. A preliminary report of these findings has been published (20). Work performed under auspices of United States Atomic Energy Commission.




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R. M. Jarrett and L. N. Edmunds Jr.
Persisting Circadian Rhythm of Cell Division in a Photosynthetic Mutant of Euglena
Science, March 27, 1970; 167(3926): 1730 - 1733.
[Abstract] [PDF]


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