Plant Physiol.
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Plant Physiology 43:1389-1394 (1968)
© 1968 American Society of Plant Biologists

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Gas-Liquid Chromatography of Trimethylsilyl Derivatives of Abscisic Acid and Other Plant Hormones 1

Larry A. Davis, D. E. Heinz and F. T. Addicott

Department of Agronomy, University of California, Davis, California 95616, Department of Consumer Sciences, University of California, Davis, California 95616

This paper describes a new method for qualitative and quantitative assay of abscisic acid and other acidic plant hormones, such as indoleacetic acid and the gibberellins, by the gas-liquid chromatography of their trimethylsilyl derivatives. Interfering substances in plant extracts were largely removed by preliminary column chromatography with carbon-celite and elution of the abscisic acid with 60% acetone, permitting direct determination of abscisic acid by gas-liquid chromatography using a flame ionization detector. (A level of 0.65 mg/kg fr wt was found.) This method enables measurement of amounts of abscisic acid as low as 0.025 µg. In impure samples collected by gas-liquid chromatography the abscisic acid recovered could be measured quantitatively by use of its ultraviolet absorption maximum at 260 mµ.

1 This research was supported in part by a grant from the Cotton Producers Institute and a contract with Department of the Army, Fort Detrick, Frederick, Maryland.






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Copyright © 1968 by the American Society of Plant Biologists