This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sarkissian, I. V. Articles by Srivastava, H. K. Search for Related Content PubMed PubMed Citation Articles by Sarkissian, I. V. Articles by Srivastava, H. K. Agricola Articles by Sarkissian, I. V. Articles by Srivastava, H. K.

Articles

On Methods of Isolation of Active, Tightly Coupled Mitochondria of Wheat Seedlings ¹

Institute of Life Science, Texas A & M University, College Station, Texas 77843

Tightly coupled mitochondria can be isolated from wheat seedlings 1) if young seedlings are used, 2) if the grinding medium is buffered and contains bovine serum albumin (BSA), 3) if the isolation procedure, including centrifugation is 10 to 11 minutes long. Mitochondria isolated in this manner oxidize -ketoglutarate, malate and NADH. Respiratory control (R.C.) values are around 6, 3, and 10, respectively. ADP:O values with those substrates are 4.0, 2.9, and 1.7, respectively. Mitochondria are stable for 3 hours. It is suggested that the technique may be used for preparation of mitochondria for comparative studies since activities of these mitochondria are highly reproducible.