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Plant Physiology 43:1415-1418 (1968)
© 1968 American Society of Plant Biologists

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Articles

The Isolation of Spinach Chloroplasts in Pyrophosphate Media 1

W. Cockburn, D. A. Walker and C. W. Baldry

Department of Biology, University of Leicester, London, S.W.7, Department of Botany, Imperial College, London, S.W.7, Tate and Lyle Research Centre, Keston, Kent, England

A simplified procedure (involving disruption in sorbitol-pyrophosphate mixtures) permits the separation of spinach chloroplasts which retain the ability to catalyze the photosynthetic assimilation of carbon dioxide and its associated oxygen evolution.

1 This work was supported by the Scientific Research Council of the U.K. The development of the oxygen electrode system was financed by a grant from the Royal Society.






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