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Plant Physiology 43:1419-1422 (1968)
© 1968 American Society of Plant Biologists

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Photosynthesis by Isolated Chloroplasts, Simultaneous Measurement of Carbon Assimilation and Oxygen Evolution 1

D. A. Walker, C. W. Baldry and W. Cockburn

Botany Department, Imperial College, London, S.W. 7, Tate and Lyle Research Centre, Keston, Kent, Department of Biology, University of Leicester, England

Photosynthetic carbon assimilation by isolated chloroplasts and its associated oxygen evolution were measured simultaneously in a single simplified reaction mixture. Each showed an initial lag prior to the attainment of the maximal rate and the photosynthetic quotient was unity during the period of illumination. Following illumination an oxygen uptake was observed in the dark which was not accompanied by any net release of newly fixed carbon dioxide.

1 This work was supported by the Scientific Research Council of the U.K. The development of the oxygen electrode system was financed by a grant from the Royal Society.






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Copyright © 1968 by the American Society of Plant Biologists