This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (24) Citing Articles via Google Scholar Google Scholar Articles by Sabnis, D. D. Articles by Jacobs, W. P. Search for Related Content PubMed PubMed Citation Articles by Sabnis, D. D. Articles by Jacobs, W. P. Agricola Articles by Sabnis, D. D. Articles by Jacobs, W. P.

Articles

Radioautographic Analysis of the Distribution of Label from ³H-Indoleacetic Acid Supplied to Isolated Coleus Internodes ¹

^a Biology Department, Princeton University, Princeton, New Jersey 08540

Isolated fifth internodes of Coleus blumei Benth. were supplied with ³H-IAA at their apical ends. Microradioautography, using thin sections (0.25-0.5 microns) of Epon embedded tissue and liquid emulsion techniques, revealed extremely specific localization of the acetone-insoluble derivatives of IAA. Changes in the patterns of labeling were followed over periods of 3, 24, and 48 hr at various distances from the source of applied auxin. Progressively basipetal labeling of the cell walls of the youngest xylem elements was evident with time. At the end of 24 hr the label was detected also within the cytoplasm or slime of phloem elements. After 24 to 48 hr, labeling of plastids was evident. The results suggest a continued incorporation of IAA derivatives into cell wall precursors.

³ The contributions of Mr. Hirshberg were incorporated in his 1968 experimental senior thesis at Princeton.

¹ Research supported by an NSF grant to W. P. Jacobs and by funds provided by the Eugene Higgins Trust Fund and facilities made available by the White-hall and John A. Hartford Foundation to the Department of Biology, Princeton University.

This article has been cited by other articles:

				W. P. Jacobs Hormone transport and action in the green shoot:long-term studies of a clonal stock of Coleus blumei(Labiatae) Am. J. Botany, October 1, 1998; 85(10): 1353 - 1363. [Abstract] [Full Text]