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Nature of the Conversion of Ficus carica Variety Kadota Ficin Component D to Component C. Some Physicochemical Properties of Components C and D ^1,2

^a Department of Food Science and Technology, University of California, Davis, California 95616

Component C can be formed from component D under the experimental conditions used during purification of Ficus carica variety Kadota latex. By use of the inhibitor, sodium p-chloromercuribenzoate, the 2 components have been purified to chromatographic homogeneity. The 2 components have identical molecular weights and amino acid composition. The only difference found between the 2 components is the presence of 3 to 6 more amide groups in component D than in component C. There also appears to be a conformational difference between the 2 since component C is not as acidic, with respect to component D, as would be expected from the comparative amide contents. Conformational differences between the 2 are also indicated by the chromatographic behavior of the 2 in the presence and absence of sodium-p-chloromercuribenzoate.

¹ This investigation was supported in part by the National Institutes of Health (GM-05216).

² Taken in part from the dissertation submitted by Donald E. Kramer to the Graduate Division, University of California Davis, in partial satisfaction of the requirements for the Ph.D. degree. A preliminary account of a portion of this work was presented at the Pacific Slope Biochemical Conference (Los Angeles, Sept. 2-3, 1965) and an abstract dealing with some phases of the work appears in Federation Proc. 26: 838 (1967).