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Plant Physiology 44:1645-1649 (1969)
© 1969 American Society of Plant Biologists

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Articles

Photoreduction of 2,6-Dichlorophenolindophenol by Diphenylcarbazide: A Photosystem 2 Reaction Catalyzed by Tris-Washed Chloroplasts and Subchloroplast Fragments

Leo P. Vernon and Elwood R. Shaw1

a Charles F. Kettering Research Laboratory, Yellow Springs, Ohio 45387

The use of diphenylcarbazide as an electron donor coupled to the photoreduction of 2,6-dichlorophenolindophenol by tris-washed chloroplasts or subchloroplast fragments provides a simple and sensitive assay for photosystem 2 of chloroplasts. By varying the concentration of tris buffer at pH 8.0 during an incubation period it is shown that the destruction of oxygen evolution activity is accompanied by a corresponding emergence of an ability to photooxidize diphenylcarbazide, as evidenced by absorbance changes due to diphenylcarbazide at 300 nm. The temperature-sensitive oxidation of diphenylcarbazide is inhibited by DCMU and by high ionic strengths. This activity appears to measure the primary photochemical reaction of photosystem 2.

1 Contribution No. 365 from the C. F. Kettering Research Laboratory. This investigation was supported in part by Research Grant GB-8434 from the National Science Foundation.




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D. D. Wykoff, J. P. Davies, A. Melis, and A. R. Grossman
The Regulation of Photosynthetic Electron Transport during Nutrient Deprivation in Chlamydomonas reinhardtii
Plant Physiology, May 1, 1998; 117(1): 129 - 139.
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Copyright © 1969 by the American Society of Plant Biologists