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Articles

Seed Germination Studies. III. Properties of a Cell-free Amino Acid Incorporating System From Pea Cotyledons; Possible Origin of Cotyledonary -Amylase ¹

^a Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48104

Pea cotyledonary -amylase increases dramatically both in specific activity and total activity between days 7 to 10 when germination occurs in the dark. This enzymatic activity does not seem to appear as a consequence of release or formation of an activator, removal of an inhibitor, dissociation of an inactive amylase complex, or proteolytic decomposition of a zymogen precursor. The possibility remains that the -amylase is newly synthesized during germination. The preparation and properties of a cell-free protein-synthesizing system from germinating pea cotyledons is described; polyuridylic acid must be added for L-phenylalanine incorporation. Active microsomal preparations can be obtained from cotyledons germinated 10 days.

¹ Supported by Grant AM-03718 from the National Institute of Arthritis and Metabolic Diseases, United States Public Health Service.