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Modification of Metabolic Pattern by Variation of Nicotinamide Adenine Dinucleotide Phosphate Level ¹

^a Biological Institute, Faculty of Science, Nagoya University, Nagoya, Japan

The experiments were designed to get some information on the metabolism controlled by variation of the NADP level, which is known to change with the variation of environmental factors.

The exogenous NADP added to the mitochondria prepared from Vigna sesquipedalis cotyledons was associated with and/or penetrated into the mitochondria. The combined NADP served in the operation of the mitochondrial NADP-isocitric acid dehydrogenase.

The variation of NADP level by exogenous NADP was observed to modify the rates of metabolic processes. The increase of exogenous NADP in Vigna hypocotyl slices lowered malic- and citric-acid contents and raised the -ketoglutaric acid content. The incorporation of ¹⁴C from acetate-2-¹⁴C into lipid, organic acid, amino acid, was promoted with the exogenous NADP. The ¹⁴C-incorporation into glycolic acid, malic acid and glutamic acid was accelerated.

In the mannitol homogenate of Vigna cotyledon, ¹⁴CO₂ evolution and ¹⁴C-incorporation into lipid, sugar, and glycolic acid from acetate-2-¹⁴C were promoted with the exogenous NADP. Endogenous citric acid content was lowered by NADP, while malic acid content was increased.

The activation of NADP-enzymes by NADP was discussed to be involved in these variations.