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Plant Physiology 44:1089-1094 (1969)
© 1969 American Society of Plant Biologists

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Articles

Long-lived Intermediates in Phytochrome Transformation II: In Vitro and In Vivo Studies 1

Winslow R. Briggsa,2

David C. Forkb

a Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138, Department of Plant Biology, Carnegie Institute of Washington, Stanford, California 94305

Conditions of illumination which cause phytochrome to cycle rapidly from PR to PFR and back lead to the accumulation in vivo of detectable amounts of long-lived intermediates on the PR to PFR pathway in oat coleoptile tissue. They appear to decay independently and in parallel to PFR. Their behavior under different intensities of illumination and exposure time suggests that they are homologous with 2 similar intermediates previously observed in vitro. Available evidence favoring this suggestion is discussed. Equivalent illumination apparently causes far higher steady state levels of absorption by intermediates in vivo than in vitro, suggestion that native phytochrome is in a different physical state in the cell than it is in solution. A difference spectrum for the intermediates in vitro between 365 and 580 nm is presented. It has a maximum at 380 nm, a minimum at 418 nm, and crossover points at 398 and 485 nm. Glycerol in the phytochrome sample enhances the signal without otherwise changing the spectrum in any way. The difference spectrum represents the difference in absorption between the combined intermediates and PFR.

2 Most of the experiments described in this paper were performed while the senior author was a member of the Department of Biological Sciences, Stanford University, Stanford, California 94305.

1 Supported by National Science Foundation Grants GB-2846 and GB-6683 and a grant from Research Corporation to W. R. B.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1969 by the American Society of Plant Biologists