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Rapid Regeneration of Protochlorophyllide₆₅₀¹

The Rockefeller University, New York, New York 10021, ^b Brookhaven National Laboratory, Upton, New York 11973

The rate of regeneration of protochlorophyllide₆₅₀ was examined spectrophotometrically after a saturating light flash using 8- to 9-day-old dark-grown bean leaves. The regeneration occurred to the extent of 15% with a half rise time of about 20 seconds. Feeding -aminolevulinic acid to the excised leaves in the dark increased protochlorophyllides₆₃₅ but not the absorption at 650 nanometers, suggesting that the holochrome was normally saturated with protochlorophyllide and that the holochrome protein was not controlled by the level of protochlorophyllide. After a light flash, the excess protochlorophyllide, formed from exogenous -aminolevulinic acid, readily combined to regenerate the 650 nanometer absorbing species; the regeneration occurred to the extent of 60 to 80% with a half rise time of about 50 seconds. Regeneration was blocked at 0°, suggesting that there was some enzymic process required for regeneration, possibly the formation of a reductant component of the protochlorophyllides₆₅₀ holochrome.

¹ This research was supported in part by Research Grant GM-04922 from the National Institutes of Health and United States Public Health Service Fellowship 1-F2-GM-36,658-01 to M. Gassman. It is dedicated to the memory of James H. C. Smith whose early studies on protochlorophyllide laid a firm foundation for work in this field.

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