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The Biosynthesis of Ethyl--glucoside in Extracts of Pea Seedlings ¹

^a Department of Botany, University of California, Davis, California 95616

An enzyme, which lacks cellobiase activity, responsible for the synthesis of ethyl--glucoside has been found in the extracts of pea hooks (1-centimeter length of the apical portion of epicotyl) and has been partially purified by ammonium sulfate fractionation. The enzyme can transfer the glucosyl moiety from a group of phenolic -glucosides to ethanol. A specific -glucosyl donor, isosuccinimide -glucoside, isolated from the extracts of pea seedlings shows the highest activity. The characteristics of the enzyme which synthesizes ethyl--glucoside and the glucosyl donor, isosuccinimide -glucoside, have been studied. The significance of this system (enzyme and isosuccinimide -glucoside) has been discussed.

¹ Supported in part by Research Grant GM-07352 from the United States Public Health Service.

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