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Plant Physiology 45:475-483 (1970)
© 1970 American Society of Plant Biologists

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The Effect of 3-(3,4-Dichlorophenyl)-1,1-dimethylurea on Chloroplast Development and Maintenance in Euglena gracilis

I. Ultrastructural Characterization of Light-Grown Cells by the Techniques of Thin Sectioning and Freeze-Etching 1

Stanley C. Holt and Arthur I. Stern

a Departments of Microbiology and Botany, University of Massachusetts, Amherst, Massachusetts 01002

The ultrastructure of light-grown Euglena gracilis var. bacillaris was examined by the techniques of thin sectioning and freeze-etching. Thin sectioning revealed the typical organelles previously observed in chemically fixed Euglena. In addition to confirming the observations on thin sections, the freeze-etch technique has revealed the presence in E. gracilis of a complex multilaminar pellicle, and an ordered arrangement to the paramylon granule. The chloroplast thylakoids are particulate and similar to those observed in higher plants.


1 This work was supported by grants to S. C. H. and A. I. S. from the United States Public Health Service (GM-14634) and from the National Science Foundation (GB-7274), respectively.




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H. N. Guttman
Internal Cellular Details of Euglena gracilis Visualized by Scanning Electron Microscopy
Science, January 22, 1971; 171(3968): 290 - 292.
[Abstract] [PDF]




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Copyright © 1970 by the American Society of Plant Biologists