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The Involvement of Glycosidases in the Cell Wall Metabolism of Suspension-cultured Acer pseudoplatanus Cells ¹

^a Department of Chemistry, University of Colorado, Boulder, Colorado 80302

Several glycosidases have been isolated from suspensioncultured sycamore (Acer pseudoplatanus) cells. These include an -galactosidase, an -mannosidase, a -N-acetyl-glucosaminidase, a -glucosidase, and two -galactosidases. The pH optimum of each of these enzymes was determined. The pH optima, together with inhibition studies, suggest that each observed glycosidase activity represents a separate enzyme. Three of these enzymes, -glucosidase, -galactosidase, and one of the -galactosidases, have been shown to be associated with the cell surface. The enzyme activities associated with the cell surface were shown to possess the ability to degrade to a limited extent isolated sycamore cell walls. It was found that the activities of -glucosidase and of one of the -galactosidases increase as the cells go through a period of growth and decrease as cell growth ceases.

¹ Research was supported in part by Grant AT(11-1)-1426 from the United States Atomic Energy Commission.

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