Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 46:240-246 (1970)
© 1970 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (157)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nadler, K.
Right arrow Articles by Granick, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nadler, K.
Right arrow Articles by Granick, S.
Agricola
Right arrow Articles by Nadler, K.
Right arrow Articles by Granick, S.
Articles

Controls on Chlorophyll Synthesis in Barley 1

Kenneth Nadler2 and S. Granick

Rockefeller University, New York, New York 10021

In 7- to 10-day-old leaves of etiolated barley (Hordeum vulgare), all of the enzymes that convert {delta}-aminolevulinic acid to chlorophyll are nonlimiting during the first 6 to 12 hours of illumination, even in the presence of inhibitors of protein synthesis. The limiting activity for chlorophyll synthesis appears to be a protein (or proteins) related to the synthesis of {delta}-aminolevulinic acid, presumably {delta}-aminolevulinic acid synthetase. Protein synthesis in both the cytosol and plastids may be required to produce nonlimiting amounts of {delta}-aminolevulinic acid. The half-life of a limiting protein controlling the synthesis of {delta}-aminolevulinic acid appears to be about 11/2 hours, when determined with inhibitors of protein synthesis. Acceleration of chlorophyll synthesis by light is not inhibited by inhibitors of nucleic acid synthesis, but is inhibited by inhibitors of protein synthesis. A model for control of chlorophyll synthesis is proposed, based on a light-induced activation at the translational level of the synthesis of proteins forming {delta}-aminolevulinic acid, as well as the short half-life of these proteins. Evidence is presented confirming the idea that the holochrome on which protochlorophyllide is photoreduced to chlorophyllide functions enzymatically.

2 The work reported is part of a thesis of K. Nadler to satisfy requirements of the doctorate degree at the Rockefeller University. More extensive references than those cited and tables of data not included in the manuscript may be obtained from the thesis. The present address of K. Nadler is: Botany Department, Michigan State University, East Lansing, Michigan 48823.

1 This investigation was supported in part by a Research Grant GM-04922 from the Division of Research Grants and Fellowships of the National Institutes of Health, United States Public Health Service, and by a Grant, GB-6818, of the National Science Foundation.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
L. A. Nogaj, A. Srivastava, R. van Lis, and S. I. Beale
Cellular Levels of Glutamyl-tRNA Reductase and Glutamate-1-Semialdehyde Aminotransferase Do Not Control Chlorophyll Synthesis in Chlamydomonas reinhardtii
Plant Physiology, September 1, 2005; 139(1): 389 - 396.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. V. Wettstein, A. Kahn, O. F. Nielsen, and S. Gough
Genetic Regulation of Chlorophyll Synthesis Analyzed with Mutants in Barley
Science, May 17, 1974; 184(4138): 800 - 802.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Meskauskiene, M. Nater, D. Goslings, F. Kessler, R. op den Camp, and K. Apel
FLU: A negative regulator of chlorophyll biosynthesis in Arabidopsisthaliana
PNAS, October 23, 2001; 98(22): 12826 - 12831.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1970 by the American Society of Plant Biologists