Plant Physiol.
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Plant Physiology 46:294-298 (1970)
© 1970 American Society of Plant Biologists

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Articles

The Fractionation of Transfer Ribonucleic Acid from Roots of Pea Seedlings 1

Larry N. Vanderhoef2 and Joe L. Key3

a Department of Botany and Plant Pathology, Purdue University, Lafayette, Indiana 47907

Isoaccepting transfer RNA species for several amino acids were fractionated by reverse phase column chromatography. Transfer RNA from dividing cells of pea (Pisum sativum) root was compared to that from nondividing cells, and no relative quantitative or qualitative differences were noted for the transfer RNA species for leucine, lysine, proline, threonine, methionine, serine, and phenylalanine. However, certain artifactual differences for serine and phenylalanine were noted. Quantitative differences were observed in tyrosyl-transfer RNA's. Ribonuclease action on tRNA did not contribute to the tRNA species observed.

2 Present address: United States Department of Agriculture Pioneering Research Laboratory, Plant Pathology, University of Wisconsin, Madison, Wis. 53706.

3 Present address: Botany Department, University of Georgia, Athens, Ga. 30601.

1 Purdue University Agricultural Experiment Station Paper 4149. The research was supported by United States Public Health Service Grant CA 10932 from the National Cancer Institute.






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Copyright © 1970 by the American Society of Plant Biologists