Plant Physiol. Drug Metab Dispos
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Plant Physiology 46:650-654 (1970)
© 1970 American Society of Plant Biologists

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Articles

Enzymic Mechanism of Starch Breakdown in Germinating Rice Seeds

III. {alpha}-Amylase Isozymes 1

Yoshimasa Tanaka, Tomoko Ito and Takashi Akazawa

a Seikagaku Seigyo Kenkyu Shisetsu, Nagoya University, School of Agriculture, Chikusa, Nagoya, Japan

The formation of amylase isozymes in germinating rice (Oryza sativa) seeds was studied by isoelectric focusing on polyacrylamide gel disc electrophoresis. Time sequence comparisons of the amylase zymogram were made between extracts from gibberellic acid-treated embryoless and embryo-attached half-endosperm of rice seeds. In both cases, 4 major and 9 to 10 minor isozyme bands were detectable at the maximal stage of the enzyme induction. However, in the embryo-attached half-seeds, bands started to diminish after the 5th day of incubation, in agreement with the results of time sequence analyses of enzyme activities. Nearly identical patterns of amylase isozyme bands on a polyacrylamide gel disc electrophoresis in combination with isoelectric focusing indicate the intrinsic role of gibberellic acid in the starch breakdown in germinating rice seeds. We tentatively assign the newly synthesized enzymes to be {alpha}-amylases based on experimental results concerning the lability of the preparation on a prolonged treatment at pH 3.3 and the stability on heat treatment for 15 minutes at 70 C.

1 This investigation was supported in part by the United States Department of Agriculture under the PL 480 Program (FG-JA-126).




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A. M. Ismail, E. S. Ella, G. V. Vergara, and D. J. Mackill
Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa)
Ann. Bot., January 1, 2009; 103(2): 197 - 209.
[Abstract] [Full Text] [PDF]


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