This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (126) Citing Articles via Google Scholar Google Scholar Articles by Pierce, W. S. Articles by Higinbotham, N. Search for Related Content PubMed PubMed Citation Articles by Pierce, W. S. Articles by Higinbotham, N. Agricola Articles by Pierce, W. S. Articles by Higinbotham, N.

Articles

Compartments and Fluxes of K⁺, NA⁺, and CL^– in Avena Coleoptile Cells ¹

^a Department of Botany, Washington State University, Pullman, Washington 99163

By the compartmental analysis method of MacRobbie and Dainty, and Pitman, estimates of K⁺, Na⁺, and Cl^– concentrations and fluxes were obtained for the cytoplasm and vacuole of coleoptile cells of oat, Avena sativa L. cv. Victory. Double labeling was used in experiments with ⁴²K plus ²²Na and with ⁴²K plus ³⁶Cl in a complete nutrient solution. At the plasmalemma, according to the Ussing-Teorell flux ratio equation, Na⁺ is pumped out and Cl^– is actively transported inward. The results with K⁺ are less conclusive, but it is probably pumped in. At the tonoplast there is an active inward transport of Na⁺ and probably of K⁺, but the status of Cl^– is uncertain, depending upon whether there is an electrical potential difference between the cytoplasm and vacuole. The results suggest that ion selectivity resides mostly in the plasmalemma. Possible errors in the estimates and interpretations are discussed.

This article has been cited by other articles:

				M. W. Szczerba, D. T. Britto, and H. J. Kronzucker Rapid, Futile K+ Cycling and Pool-Size Dynamics Define Low-Affinity Potassium Transport in Barley Plant Physiology, August 1, 2006; 141(4): 1494 - 1507. [Abstract] [Full Text] [PDF]

				M. Murthy and M. Tester Cation currents in protoplasts from the roots of a Na+ hyperaccumulating mutant of Capsicum annuum J. Exp. Bot., March 1, 2006; 57(5): 1171 - 1180. [Abstract] [Full Text] [PDF]

				D. T. Britto, M. W. Szczerba, and H. J. Kronzucker A new, non-perturbing, sampling procedure in tracer exchange measurements J. Exp. Bot., March 1, 2006; 57(6): 1309 - 1314. [Abstract] [Full Text] [PDF]

				E. Martinoia, A. Massonneau, and N. Frangne Transport Processes of Solutes across the Vacuolar Membrane of Higher Plants Plant Cell Physiol., November 1, 2000; 41(11): 1175 - 1186. [Abstract] [Full Text] [PDF]

				M. M. Lasat, N. S. Pence, D. F. Garvin, S. D. Ebbs, and L. V. Kochian Molecular physiology of zinc transport in the Zn hyperaccumulator Thlaspi caerulescens J. Exp. Bot., January 1, 2000; 51(342): 71 - 79. [Abstract] [Full Text] [PDF]

				M. M. Lasat, A. J.M. Baker, and L. V. Kochian Altered Zn Compartmentation in the Root Symplasm and Stimulated Zn Absorption into the Leaf as Mechanisms Involved in Zn Hyperaccumulation in Thlaspi caerulescens Plant Physiology, November 1, 1998; 118(3): 875 - 883. [Abstract] [Full Text]

				J. Ryu, S Takagi, and R Nagai Stationary organization of the actin cytoskeleton in Vallisneria: the role of stable microfilaments at the end walls J. Cell Sci., January 4, 1995; 108(4): 1531 - 1539. [Abstract] [PDF]