Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 47:1-6 (1971)
© 1971 American Society of Plant Biologists

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Articles

Host-Pathogen Interactions

II. Parameters Affecting Polysaccharide-degrading Enzyme Secretion by Colletotrichum lindemuthianum Grown in Culture 1

Patricia D. English2, Joseph Byrne Jurale and Peter Albersheim

a Department of Chemistry, University of Colorado, Boulder, Colorado 80302

The effect of a number of physiological variables on the secretion of polysaccharide-degrading enzymes by culture-grown Colletotrichum lindemuthianum (Saccardo and Magnus) Scribner was determined. The number of spores used to inoculate cultures grown on isolated bean hypocotyl cell walls affects the time after inoculation at which enzyme secretion occurs, but has no significant effect on the maximal amount of enzyme ultimately secreted. Cell walls isolated from bean leaves, first internodes, or hypocotyls (susceptible to C. lindemuthianum infection), when used as carbon source for C. lindemuthianum growth, stimulate the fungus to secrete more {alpha}-galactosidase than do cell walls isolated from roots (resistant to infection). The concentration of carbon source used for fungal growth determines the final level of enzyme activity in the culture fluid. The level of enzyme secretion is not proportional to fungal growth; rather, enzyme secretion is induced. Maximal {alpha}-galactosidase activity in the culture medium is found when the concentration of cell walls used as carbon source is 1% or greater. A higher concentration of cell walls is necessary for maximal {alpha}-arabinosidase activity. Galactose, when used as the carbon source, stimulates {alpha}-galactosidase secretion but, at comparable concentrations, is less effective in doing so than are cell walls. Polysaccharide-degrading enzymes are secreted by C. lindemuthianum at different times during growth of the pathogen on isolated cell walls. Pectinase and {alpha}-arabinosidase are secreted first, followed by {beta}-xylosidase and cellulase, then {beta}-glucosidase, and, finally, {alpha}-galactosidase.

2 Predoctoral Fellow of the United States Department of Health, Education and Welfare under Title IV of the National Defense Education Act.

1 This research was supported by United States Atomic Energy Commission Grant AT(11-1)-1426.






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Copyright © 1971 by the American Society of Plant Biologists