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Plant Physiology 47:119-123 (1971)
© 1971 American Society of Plant Biologists

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Articles

The Fate of L-Phenylalanine Fed to Germinating Pea Seeds, Pisum sativum (L.) var. Alaska, during Imbibition 1

Constance Nozzolillo, K. B. Paul2 and C. Godin3

a Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

Radioactive L-phenylalanine-l-14C or -U-14C was fed to pea seeds during imbibition. More than 95% was imbibed. Less than 1% of the radioactivity was respired as CO2. Of the radioactivity taken into the embryos, 80% was still in the cotyledons by 3 days. About half of this was unchanged phenylalanine: 5% free, 10 to 20% in soluble proteins, 1 to 6% in cell wall proteins, and 14% released by mild acid hydrolysis. No other radioactive amino acid was found. About 0.3% of the radioactivity was identified as free caffeic, ferulic, and coumaric acids or their glycosides, and a further 5% was released by mild acid hydrolysis into a phenolic acid fraction. About half of the radioactivity in the cotyledons was lost in the fractionation procedures.

About 20% of the radioactivity was found in the shootroot axes to which it had been translocated. Phenylalanine accounted for nearly all the identifiable radioactivity: about 1% free, 1 to 3% in soluble proteins, 1 to 5% in cell wall proteins, and 1% bound to insoluble fractions from which it was released by mild acid hydrolysis. About 1% was released into a phenolic acid fraction. About half of the radioactivity in the shoot-root axes was lost in the fractionation procedures.

2 Present address: Milbank Hall, Tuskegee Institute, Alabama 36088.

3 Present address: Départment de Biochimie, Université Laval, Ste. Fóy, Québec, Canada.

1 This study was largely supported by grants from the National Research Council of Canada.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1971 by the American Society of Plant Biologists