Plant Physiol. Drug Metab Dispos
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Plant Physiology 47:832-835 (1971)
© 1971 American Society of Plant Biologists

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Articles

The Effect of Calcium and Inhibitors on Corn Mitochondrial Respiration 1

Raymond J. Miller and D. E. Koeppe

a Department of Agronomy, University of Illinois, Urbana, Illinois 61801

The effects of KCN, antimycin A, malonate, rotenone, and amytal on the oxidation of malate, succinate, and extramitochondrial reduced nicotinamide adenosine dinucleotide (NADH) by corn mitochondria were studied. Potassium cyanide and antimycin A inhibited the oxidation of all three substrates. Rotenone and amytal inhibited only the oxidation of malate, and malonate inhibited only the oxidation of succinate. Rotenone, amytal, and malonate did not inhibit the oxidation of extramitochondrial NADH. The calcium stimulation of the oxidation of extramitochondrial NADH was prevented by KCN and antimycin A but not by amytal, rotenone, or malonate. It is suggested that corn mitochondria possess a flavoprotein specific for extramitochondrial NADH and that this flavoprotein is sensitive to divalent cations.


1 Supported in part by a grant from the Research Board, University of Illinois, and by funds from the Illinois Agriculture Experiment Station.







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Copyright © 1971 by the American Society of Plant Biologists