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Articles

Metabolism of Separated Leaf Cells

II. Uptake and Incorporation of Protein and Ribonucleic Acid Precursors by Tobacco Cells ¹

^a Departments of Agricultural Biochemistry and of Chemistry, University of Arizona, Tucson, Arizona 85721

Enzymatically separated tobacco leaf cells took up amino acids, uracil, and uridine from the incubation medium and incorporated them into proteins and RNA, respectively, at a linear rate for approximately 30 hours. Both uptake and incorporation were light-dependent, although cells prepared from preilluminated plants or preillumination of cells allowed some uptake and incorporation to occur in the dark. The light was necessary to satisfy a photosynthetic requirement, but could be replaced in part by ATP in the medium.

Several lines of evidence support the conclusion that the rate of uptake of amino acids, uracil, and uridine was dependent upon the subsequent incorporation of these compounds into macromolecules.

¹ This work was supported by Grants GB 25873 and GB 27453 from the National Science Foundation, by Atomic Energy Commission Contract AT (11-1)-873, and by the Agricultural Division, Monsanto Co. University of Arizona Agricultural Experiment Station Journal Paper 1732.