Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 48:9-13 (1971)
© 1971 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (100)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jensen, R. G.
Right arrow Articles by Zaitlin, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jensen, R. G.
Right arrow Articles by Zaitlin, M.
Agricola
Right arrow Articles by Jensen, R. G.
Right arrow Articles by Zaitlin, M.
Articles

Metabolism of Separated Leaf Cells

I. Preparation of Photosynthetically Active Cells from Tobacco 1

R. G. Jensen, R. I. B. Francki2 and M. Zaitlin

a Departments of Chemistry and Agricultural Biochemistry, University of Arizona, Tucson, Arizona 85721

Suspensions of mesophyll cells, prepared from tobacco leaves by treatment with pectinase, fixed CO2 by photosynthesis. The products of carbon assimilation were similar for both cells and intact tissue. The cells sustained a constant fixation rate for 20 to 25 hours. For optimal CO2 fixation, enzymatic maceration of the tissue was accomplished in 0.8 M sorbitol, but photosynthesis was optimal in 0.6 M sorbitol at pH 7 to 7.5. A hypertonic environment during maceration, which results in cell plasmolysis, is essential to maintain intact plasmalemmas and hence photosynthetically active cells. For sustained CO2 fixation, light intensities below 500 foot-candles were required. Higher light intensities (to 1000 foot-candles) gave high initial rates of CO2 fixation, but the cells bleached and were inactive on prolonged incubation. At pH 7.0 the bicarbonate concentration at maximal velocity of CO2 fixation was about 1.5 mM and the apparent Km for bicarbonate was 0.2 mM.

2 On leave from the Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, South Australia, and supported in part by the Australian-American Educational Foundation.

1 This work was supported in part by Grants GB 27453 and GB 25873 from the National Science Foundation, by Atomic Energy Commission Contract AT (11-1)-873, and by the Agricultural Division, Monsanto Company. The University of Arizona Agricultural Experiment Station Journal Paper 1731.




This article has been cited by other articles:


Home page
 ScienceHome page
A. Siegel, D. Lightfoot, O. G. Ward, and S. Keener
DNA Complementary to Ribosomal RNA: Relation between Genomic Proportion and Ploidy
Science, February 16, 1973; 179(4074): 682 - 683.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1971 by the American Society of Plant Biologists