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Articles

The Necessity for -Carotene in the 518 Nanometer Absorbance Change ¹

^a Johnson Research Foundation, The University of Pennsylvania, Philadelphia, Pennsylvania 19104

The identity of the pigment responsible for the light-induced 518 nanometer absorbance change was investigated by extraction and reconstitution of spinach chloroplasts. Heptane extraction of carotene and quinones from lyophilized chloroplasts removes absorbance changes at 518 and 475 nanometers activated by both laser flash and continuous illumination. Some electron transport activity is always present, even in carotene- and quinone-depleted chloroplasts, but the light-induced pH increase disappears following the first extraction step. Readdition of pure -carotene partially restores the 518 and 475 nanometer absorbance changes.

¹ This research was supported by United States Public Health Service Training Grant GM2G27709. Submitted in partial fulfillment of requirements for the degree of Doctor of Philosophy to the Graduate School of Arts and Sciences of the University of Pennsylvania.