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Plant Physiology 49:348-352 (1972)
© 1972 American Society of Plant Biologists

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Articles

Hormonal Control of Polyribosome Formation in Barley Aleurone Layers 1

Warren H. Evins2 and J. E. Varner

a Department of Biochemistry and Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48823

The addition of abscisic acid to barley (Hordeum vulgare L. cv. Himalaya) aleurone layers at the same time as gibberellic acid completely prevents the gibberellin-induced increases in the percentage of polysomes, the formation of polyribosomes, and the synthesis of {alpha}-amylase, even when the molar concentration of gibberellic acid is four times greater than the concentration of abscisic acid. The addition of abscisic acid to aleurone cells producing {alpha}-amylase (midcourse addition) inhibits the further synthesis of {alpha}-amylase and decreases the percentage of polysomes but does not change the number of ribosomes per cell.

The removal of gibberellic acid from aleurone layers during the midcourse of {alpha}-amylase production arrests {alpha}-amylase synthesis and decreases the percentage of polysomes. Readdition of gibberellic acid causes the reinitiation of the synthesis of {alpha}-amylase and a return of the percentage of polysomes to the original level.

The incubation of aleurone layers with 5-fluorouracil inhibits the secretion of {alpha}-amylase. The changes in polysomes isolated from cells treated with either fluorouracil or actinomycin D correlate with the changes in enzyme synthesis caused by the addition of these inhibitors.

Gibberellic acid and abscisic acid affect both the conversion of monosomes to polysomes and the synthesis of new ribosomes. The gibberellin-stimulated increases in the number of ribosomes and the percentage of polysomes are probably a prerequisite for the hormone induction of enzyme synthesis.

2 Present address: The Rockefeller University, New York, N. Y. 10021.

1 The work was supported by the United States Atomic Energy Commission (contract AT(11-1)-1338) and a National Science Foundation grant (GB-8774) to J. E. V. This paper is based on a dissertation submitted by W. H. E. in partial fulfillment of the requirements for a Ph.D. degree, Department of Biochemistry, Michigan State University. It is Michigan Agricultural Experimentation journal article No. 5476.




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D. W. FOUNTAIN, D. E. FOARD, W. D. REPLOGLE, and W. K. YANG
Lectin Release by Soybean Seeds
Science, September 16, 1977; 197(4309): 1185 - 1187.
[Abstract] [PDF]


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